artificialFRW1Germline - FWR1 artificial dataset generator
Description¶
A function to artificially create an IGHV reference set with framework1 (FWR1) primers (see Details).
Usage¶
artificialFRW1Germline(
germline_set,
mask_primer = T,
trimm_primer = F,
quite = F
)
Arguments¶
- germline_set
- A germline set distance matrix created by
ighvDistance
. - mask_primer
- Logical (TRUE by default). If to mask with Ns the region of the primer from the germline sequence
- trimm_primer
- Logical (FALSE by default). If to trim the region of the primer from the germline sequence. If TRUE then, mask_primer is ignored.
- quite
- Logical (FALSE by default). Do you want to suppress informative messages
Value¶
A list
with the input germline set allele and the trimmed/masked sequences.
Details¶
The FRW1 primers used in this function were taken from the BIOMED-2 protocol. For more information on the protocol and primer design go to: van Dongen, J., Langerak, A., Brüggemann, M. et al. Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia 17, 2257–2317 (2003). https://doi.org/10.1038/sj.leu.2403202Van Dongen, J. J. M., et al. “Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936.” Leukemia 17.12 (2003): 2257-2317.